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Titlebook: Working with Stem Cells; Henning Ulrich,Priscilla Davidson Negraes Book 2016 Springer International Publishing Switzerland 2016 Nanostruct

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Henning Ulrich,Priscilla Davidson NegraesThe most updated methods and technologies of how to work on stem cell culture and differentiation, developed and optimized by whom uses them every day.Easy language, making reading and learning simple
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978-3-319-80841-3Springer International Publishing Switzerland 2016
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Book 2016owing field that offers the potential to comprehend mechanisms of neural development and tissue regeneration and maintenance as well as the development-related human diseases and the establishment of novel therapies. Questions of basic biology can also be exploited by this cutting-edge approach. .. .
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Spontaneous Generation of Patient-Specific Retinal Pigment Epithelial Cells Using Induced Pluripotese that produce a facsimile of the developing eye .. This chapter introduces the importance of the Retinal Pigment Epithelium (RPE) in maintaining visual function. We then focus on methods developed by our group to produce RPE from patient skin samples using human induced pluripotent stem cell technology (iPSC).
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Human Embryonic Stem Cell Line Derivation, lines using conventional procedures and in a xeno-free culture condition, as well as discuss the potential therapeutic use of these cells in regenerative medicine and in pharmaceutical drug screening.
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Derivation of Dopaminergic Neurons from Human Pluripotent Stem Cells Using a Defined System and/or ients with neurodegenerative disorders. Here, we describe two established protocols for the efficient derivation of dopaminergic neurons from PSCs using defined media and/or small molecules. Dopaminergic neurons can be efficiently generated using either of these protocols on a large scale.
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Differentiation of hiPSC-Derived Cardiomyocytes, of the Wnt signaling pathway, which is critical for early cardiac development. In this chapter we explore adaptations made to boost chemically defined cardiac differentiation protocols to further enhance their effectiveness and reproducibility across independent hiPSC clones and lines.
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Stem Cells and Tissue Engineering,iation in functional cells for efficient tissue repair. Additionally, we present a protocol to induce pluripotent stem cells to differentiate into bone cells, in order to generate an . material for bone repair.
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