Titlebook: Wheat Biotechnology; Methods and Protocol Prem L. Bhalla,Mohan B. Singh Book 2017 Springer Science+Business Media LLC 2017 Biotechnological

habitat

Wheat Improvement in India: Present and Futuresources, environmental fluctuation, and increased risk of epidemic outbreaks, the task of increasing wheat production has become daunting. The factors responsible for first green revolution seem to be exhausting rapidly, and there is an immediate need to develop the technologies which can not only i

歪曲道理

动机

Allergenicity Assessment of Transgenic Wheat Lines In Silicoy modified (GM) food crops improve crop characteristics like nutritional value, taste, and texture, and endow plants with resistance against fungus, pests, and insects. Despite such potential benefits, there are concerns regarding possible adverse effects of GM crops on human health, animals and the

载货清单

Agribusiness Perspectives on Transgenic Wheatticultural crops and foodstuffs which are genetically modified have potentially serious implications. It makes the chance less than otherwise would be the case that agribusiness value chains in the future will meet the growing demand around the world for more and different foods from more and wealth

Dendritic-Cells

-Mediated Transformation of Wheat Using Immature Embryosr functional genomics as well as for wheat breeding. In the protocol described here, immature embryos of the spring-type model genotype Bobwhite SH 98 26 have been used. Preculture and temperature pretreatment of these explants have led to the reproducible generation of transgenic plants at efficien

Innocence

死亡率

使人入神

Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systemss-associated system (CRISPR/Cas9) have provided powerful reverse genetic approaches to the targeted modification of genomes in numerous organisms. Both systems have been employed to generate loss-of-function alleles in bread wheat, by targeting multiple and single copies of genes. Here we present pr

CRUC

Design and Assembly of CRISPR/Cas9 Reagents for Gene Knockout, Targeted Insertion, and Replacement igeted and specific modifications in this crop plant. This protocol demonstrates the construction of reagents using a comprehensive genome engineering kit to create single and multiple gene “knockouts,” site-specific chromosome deletions and gene replacement or “knockins” including the use of geminiv

Mosaic

Doubled Haploid Transgenic Wheat Lines by Microspore Transformationablished gene-delivery methods of particle bombardment, electroporation, and cocultivation with . were optimized on androgenic microspores or derived tissues. In the biolistic gene delivery method 35–40 days old haploid microspore embryoids were used for genetic transformation, whereas freshly isola