Titlebook: Western Blotting for the Non-Expert; Biji T. Kurien Book 2021 Springer Nature Switzerland AG 2021 Diffusion Protein Transfer.Electrophores

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Nitrocellulose Membrane Art,Kurien to use nitrocellulose membrane as the new canvas for art. Their work showed that nitrocellulose membrane could serve as a canvas for art using alkaline phosphatase as the invisible ink and then detecting the art magically with the NBT/BCIP detection system.

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Fingerprinting on Nitrocellulose and Polyvinylidene Difluoride Membranes, describes Kurien and coauthors’ work that showed the use of alkaline phosphatase to transfer prints from fingers or palm to nitrocellulose or polyvinylidene difluoride membranes. One can detect such prints with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate.

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Western Blotting: How It Began,the Nobel Prize for describing the molecular structure of DNA. The genetic code had been deciphered. Nobel laureate Dr. Frederick Sanger developed a method in the early 1950s to determine the amino acid units that made up a protein structure. Now, he was set to invent an elegant method to sequence D

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Basics of Western Blotting,procedure known as protein blotting or Western blotting. Cells obtained from tissues or cells grown outside the human body in a plastic flask are first busted to release its contents. The cell contents, which include a mixture of proteins, are first separated from each other according to the protein

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Selecting Antibodies for Western Blotting, amounts of proteins with the help of suitable antibodies. The main feature of an effective immunoblot is the particular interaction between an antigen and an antibody. Therefore, the selection of the right antibodies plays a crucial role in this procedure. Both polyclonal or monoclonal antibodies a

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Blocking Membranes at Places That Do Not Have Transferred Proteins,ne not occupied by the proteins transferred to the membrane from the SDS-PAGE gel. In other words, they block off the empty areas on the membrane so that the primary antibody will not bind to those empty areas of the membrane in addition to its specific protein transferred from the gel to the membra