Titlebook: West Nile Virus; Methods and Protocol Fengwei Bai Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr

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1064-3745 ation advice from the experts.This volume provides researchers with the most updated understanding of the basics of West Nile Virus (WNV). Chapters focus on the biology, virology, epidemiology, pathogenesis, as well as the step-by-step molecular, cellular, and statistical methods to study WNV infect

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Book 2023n troubleshooting and avoiding known pitfalls.. . Cutting-edge and comprehensive, .West Nile Virus: Methods and Protocols .aims to be a valuable resource for all researchers interested in learning more about this important and developing field..

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Introduction to West Nile Virus, the USA in 1999, WNV has become the most common mosquito-borne virus in North America. Despite the intensive research over 20 years, there are still no approved vaccines or specific treatments for humans, and it remains an urgent need to understand the pathogenesis of WNV and develop specific therapeutics and vaccines.

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Protocol to Study West Nile Virus Infection in Brain Slices In Vitro,t possible in in vitro cell lines. Here, we describe the method of preparing ex vivo slice culture from the mouse cerebellum and the protocol of studying the effects of West Nile virus infection on cerebellar cells.

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Studying Virus-Host Interactions with CRISPR Technology,erful and convenient tool to perturbate host gene expression, allowing for unbiased, genome-wide screens of host factors for virus infection. Here we describe the necessary steps for performing a CRISPR knockout screen, which can also be applied to other viruses, to identify host factors critical for WNV infection.

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Development of Antibody-Based Therapeutics Against West Nile Virus in Plants,cording to the Center for Disease Control and Prevention, more than 51,000 cases of WNV infection and nearly 2400 cases of WNV-related death were reported in the USA from 1999 to 2019. The estimated economic impact of WNV infections is close to 800 million dollars in the USA from 1999 to 2012 (Barrett, Am J Trop Med Hyg 90:389, 2014).

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