Titlebook: 3D Sponge-Matrix Histoculture; Methods and Protocol Robert M. Hoffman Book 2018 Springer Science+Business Media, LLC, part of Springer Natu

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https://doi.org/10.1007/978-3-322-83634-2ution of cancer cells in Gelfoam. histoculture was similar to in vivo tumors, whereby only the surface cells proliferate and interior cells are quiescent in G./G.. In contrast, in 2D cancer-cell culture, most of the cells are always cycling. The cancer cells responded similarly to toxic chemotherapy

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https://doi.org/10.1007/978-3-322-83816-2al papilla (DP). The BA appears to be origin of HAP stem cells. Long-term Gelfoam. histoculture was established of whiskers isolated from transgenic mice, in which there is nestin-driven green fluorescent protein (ND-GFP). HAP stem cells trafficked from the BA toward the DP area and extensively grew

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https://doi.org/10.1007/978-3-322-83816-2cell interactions by mirroring their original spatial relationship within body tissues. Gelfoam. histoculture can be employed to model host-pathogen interactions mimicking in vivo conditions in vitro. In the present chapter, we describe a protocol to process and infect lymphoid tissue explants with

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