Titlebook: 3D Cell Culture; Methods and Protocol Zuzana Koledova Book 2017 Springer Science+Business Media LLC 2017 Hydrogels.3D Organoids.Scaffolds.O

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https://doi.org/10.1007/978-3-322-83299-3nnovative 3D-dynamic culture technology, based on the use of the Rotary Cell Culture System (RCCS™) bioreactor. Here, we describe, step by step, the procedures we have employed to establish two human MM ex vivo models, i.e., the culture of human BM-derived isolated cells and of MM tissues from patients.

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https://doi.org/10.1007/978-3-322-83299-3 and epidermal layer and thus can be used as more complex models for the investigation of processes such as wound healing, skin development, or infectious diseases. In this chapter, we describe the generation and culture of an FTSE, based on a collagen type I matrix and provide troubleshooting tips for commonly encountered technical problems.

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Ökologische Kommunikation in Deutschlandnisms driving prostate cancer as well as to screen for more efficient drugs that can be used for treatment. In this chapter we describe a 3D hanging drop system that can be used to culture prostate cancer organoids as tumor epithelial monocultures and as epithelial–stromal cocultures.

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