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Titlebook: 3D Cell Culture; Methods and Protocol Zuzana Koledova Book 2017 Springer Science+Business Media LLC 2017 Hydrogels.3D Organoids.Scaffolds.O

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发表于 2025-3-21 17:27:27 | 显示全部楼层 |阅读模式
期刊全称3D Cell Culture
期刊简称Methods and Protocol
影响因子2023Zuzana Koledova
视频video
发行地址Includes cutting-edge methods and protocols for the study of 3D cell cultures.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from experts.
学科分类Methods in Molecular Biology
图书封面Titlebook: 3D Cell Culture; Methods and Protocol Zuzana Koledova Book 2017 Springer Science+Business Media LLC 2017 Hydrogels.3D Organoids.Scaffolds.O
影响因子This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Comprehensive and cutting-edge, .3D Cell Culture: Methods and Protocols. aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures..Chapter 15 of this book is available open access under a CC BY 4.0 license..
Pindex Book 2017
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发表于 2025-3-21 20:22:49 | 显示全部楼层
Methods in Molecular Biologyhttp://image.papertrans.cn/012/image/100701.jpg
发表于 2025-3-22 03:07:44 | 显示全部楼层
https://doi.org/10.1007/978-1-4939-7021-6Hydrogels; 3D Organoids; Scaffolds; Organotypic cultures; Micropatterning; Microfluidics; Bioprinting; Thre
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978-1-4939-8370-4Springer Science+Business Media LLC 2017
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发表于 2025-3-22 15:29:30 | 显示全部楼层
https://doi.org/10.1007/978-3-322-83139-2–cell and cell–extracellular matrix interactions, 3D cell culture enables proper structural architecture and differentiated function of normal tissues or tumors in vitro. Thereby 3D cell culture realistically models in vivo tissue conditions and processes, and provides in vivo like responses. Since
发表于 2025-3-22 19:03:08 | 显示全部楼层
https://doi.org/10.1007/978-3-322-83139-2bserved in vivo. Decellularization of tissues and organs has been shown to be an effective method for the removal of potentially immunogenic constituents while preserving essential growth factors and extracellular matrix (ECM) proteins necessary for proper cell function. Enzymatic digestion of decel
发表于 2025-3-22 23:02:20 | 显示全部楼层
https://doi.org/10.1007/978-3-322-83244-3 with native extracellular matrix (ECM) proteins, nonnative biopolymers, or synthetic polymers are often used to study these processes in vitro in 3D cell culture experiments. However, each of these materials systems present major limitations when used in mechanobiological studies. While native ECM-
发表于 2025-3-23 01:32:39 | 显示全部楼层
https://doi.org/10.1007/978-3-322-83244-3of advanced three dimensional (3D) culture models. Herein, we report methods for the PEG-GAG gel-based 3D co-cultivation of human umbilical vein endothelial cells, mesenchymal stromal cells, and different cancer cell lines. The resulting constructs allow for the exploration of interactions between s
发表于 2025-3-23 07:30:52 | 显示全部楼层
https://doi.org/10.1007/978-3-322-83253-5sical cell reprogramming process. Surprisingly, the contribution of the three-dimensional (3D) microenvironment to iPSC generation has been largely overlooked. Here we describe a protocol for the generation of iPSCs in defined poly(ethylene glycol) (PEG)-based hydrogels that, besides allowing higher
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