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Titlebook: Drosophila; Methods and Protocol Christian Dahmann Book 2008 Humana Press 2008 Homologous recombination.Microarray.Model organisms.cell lin

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发表于 2025-3-21 19:49:14 | 显示全部楼层 |阅读模式
书目名称Drosophila
副标题Methods and Protocol
编辑Christian Dahmann
视频video
概述Protocols are written by the scientists who invented the methods.Includes methods for homologous recombination in Drosophila.Includes methods for live imaging in embryos, larvae, and pupae
丛书名称Methods in Molecular Biology
图书封面Titlebook: Drosophila; Methods and Protocol Christian Dahmann Book 2008 Humana Press 2008 Homologous recombination.Microarray.Model organisms.cell lin
描述Drosophila melanogaster has been the model system of choice for many inv- tigators over the past hundred years. Due to its long-standing tradition as a model organism, many techniques used in Drosophila have been established and c- tinue to be developed to answer a variety of scientific questions. The recent inv- tion of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination are two important demonst- tions of the ongoing efforts to broaden the technical repertoire at hand for researchers working with Drosophila. This volume is a collection of protocols covering both standard techniques as well as novel methods. An introductory chapter highlights the importance of Drosophila as a model system for the dev- opment of Biology in the 20th century and review chapters provide concise and up-to-date overviews on selected experimental systems. This book makes no attempt to be comprehensive. A number of frequently used standard techniques were selected and are described in-depth to allow novices to get started with Drosophila. In addition, detailed protocols of recently developed methods that we felt will become of broad use in the D
出版日期Book 2008
关键词Homologous recombination; Microarray; Model organisms; cell lines; genes; hybridization; molecular biology
版次1
doihttps://doi.org/10.1007/978-1-59745-583-1
isbn_softcover978-1-61737-791-4
isbn_ebook978-1-59745-583-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2008
The information of publication is updating

书目名称Drosophila影响因子(影响力)




书目名称Drosophila影响因子(影响力)学科排名




书目名称Drosophila网络公开度




书目名称Drosophila网络公开度学科排名




书目名称Drosophila被引频次




书目名称Drosophila被引频次学科排名




书目名称Drosophila年度引用




书目名称Drosophila年度引用学科排名




书目名称Drosophila读者反馈




书目名称Drosophila读者反馈学科排名




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Research Notes in Neural Computingarchers as yet unfamiliar with . genetics to take advantage of all the benefits of this mutagenesis method, which include its wide and largely unbiased coverage of the genome, the high mutation frequency, and the variety of null, hypomorphic, conditional (e.g., temperature sensitive), or domain spec
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Colin G. Ellard,Melvyn A. Goodale the benefit that the mutations may be introduced with no other remnant of the targeting procedure. This “surgical” approach will appeal to investigators who desire minimal perturbation to the genome, such as single nucleotide mutation..Although both approaches appear to be approximately equally eff
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Video in Psychiatrie und Psychotherapie, there has been a long-standing motivation to follow cellular and synaptic events in vivo. However, to date few preparations have been studied, and often only with great difficulty. New perspectives in this field are opened up by the continuous development of powerful, genetically encoded fluoresce
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1064-3745 were selected and are described in-depth to allow novices to get started with Drosophila. In addition, detailed protocols of recently developed methods that we felt will become of broad use in the D978-1-61737-791-4978-1-59745-583-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
发表于 2025-3-22 17:16:26 | 显示全部楼层
The Use of P-Element Transposons to Generate Transgenic Flies,, and a microscope, proper handling of the . embryos before and after microinjection is the key step to the generation of transgenic flies. Pioneer work in . also served as a general guideline for the transformation of other insect species including those with medical and agricultural importance.
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EMS Screens,archers as yet unfamiliar with . genetics to take advantage of all the benefits of this mutagenesis method, which include its wide and largely unbiased coverage of the genome, the high mutation frequency, and the variety of null, hypomorphic, conditional (e.g., temperature sensitive), or domain spec
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Methods for Homologous Recombination in ,, the benefit that the mutations may be introduced with no other remnant of the targeting procedure. This “surgical” approach will appeal to investigators who desire minimal perturbation to the genome, such as single nucleotide mutation..Although both approaches appear to be approximately equally eff
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