Overview: Presents state of the art information on NMR spectroscopy, and its place in the broader field of biophysics.No other monograph presents such a wide range of topics, including NMR spectroscopy, proteinSingle-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1–3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4–6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total inter
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