SPARK
发表于 2025-3-21 19:22:30
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jumble
发表于 2025-3-21 23:07:31
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繁重
发表于 2025-3-22 00:53:30
Robert D. Poodiack,William E. Woodth at least five consecutive nucleotides of local identity. Since DNA shuffling was originally developed, many variations on the method have been published. In particular, the use of restriction enzymes in the fragmentation step allows for greater customization of fragment lengths than DNase I diges
Muffle
发表于 2025-3-22 05:58:57
Robert D. Poodiack,William E. Woodf the DNA library nor the number of DNA changes has any limits. Furthermore, with the costs of synthetic DNA dropping every year, after an initial investment is made in the oligonucleotides, these can be exchanged for alternative ones with different sequences at any point in the process, fully explo
拍下盗公款
发表于 2025-3-22 12:02:51
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污点
发表于 2025-3-22 13:30:40
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污点
发表于 2025-3-22 20:42:23
1064-3745 design and analysis of mutant libraries. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists o978-1-4939-5329-5978-1-4939-1053-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
Jogging
发表于 2025-3-22 23:48:10
Random Mutagenesis by Error-Prone Pol Plasmid Replication in ,nesis method that is based on error-prone replication of a ColE1 plasmid bearing the gene of interest. Compared to other in vivo mutagenesis methods, this plasmid-targeted approach allows increased mutation loads and facilitates iterative selection approaches. We also describe the mutation spectrum
衰老
发表于 2025-3-23 05:20:12
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Irascible
发表于 2025-3-23 09:20:21
Restriction Enzyme-Mediated DNA Family Shufflingth at least five consecutive nucleotides of local identity. Since DNA shuffling was originally developed, many variations on the method have been published. In particular, the use of restriction enzymes in the fragmentation step allows for greater customization of fragment lengths than DNase I diges