| 书目名称 | Directed Evolution Library Creation |
| 副标题 | Methods and Protocol |
| 编辑 | Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | .Directed Evolution Library Creation: Methods and Protocols, Second Edition .presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm. Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore structure-function relationships, and has supported the rapid development of the field of synthetic biology over the last decade. Divided into three convenient sections, topics include point mutagenesis strategies, recombinatorial methods wherein genetic diversity is sourced from multiple parental genes that are combined via either homology-dependent or -independent techniques and a variety of computational methods to guide the design and analysis of mutant libraries. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists o |
| 出版日期 | Book 2014Latest edition |
| 关键词 | deletion mutations; directed evolution; gene variant libraries; insertion mutations; mutagenesis; genetic |
| 版次 | 2 |
| doi | https://doi.org/10.1007/978-1-4939-1053-3 |
| isbn_softcover | 978-1-4939-5329-5 |
| isbn_ebook | 978-1-4939-1053-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media New York 2014 |
发表于 2025-3-21 19:22:30
