ingenue
发表于 2025-4-1 01:50:38
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肌肉
发表于 2025-4-1 08:55:40
Transposon-Based Approaches for Generating Novel Molecular Diversity During Directed Evolutionon endonuclease generates blunt-end random breaks at a frequency of one per target gene and the concomitant loss of 3 bp. Self-ligation or insertion of another DNA cassette results in the sampling of trinucleotide deletion or trinucleotide substitution/domain insertion, respectively.