教育学 发表于 2025-3-23 12:42:28

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令人发腻 发表于 2025-3-23 14:37:58

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Malaise 发表于 2025-3-23 21:58:23

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transdermal 发表于 2025-3-23 22:55:11

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OGLE 发表于 2025-3-24 05:42:28

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intellect 发表于 2025-3-24 08:08:43

Methods: Named Message Sequencescols. SeSaM is advantageous with respect to (1) elimination of mutagenic “hot spots”, (2) increase in frequency of subsequent nucleotide substitutions, (3) control over the mutational bias through the utilization of universal base analogs, and, consequently, (4) the prospect of generating transversi

抑制 发表于 2025-3-24 12:37:06

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逢迎白雪 发表于 2025-3-24 17:36:59

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Flat-Feet 发表于 2025-3-24 21:55:53

https://doi.org/10.1007/978-1-4302-0037-6e amino acid positions from the chosen enzyme are assigned to multi-residue sites (i.e., groups of amino acids or “multisites”). Then, the residues in each multisite are mutated with a user-defined randomization scheme to all canonical amino acids or a reduced amino acid alphabet. Subsequently, the

critique 发表于 2025-3-25 01:06:48

Installation and Creating a Robotonal diversities while avoiding high loads of deleterious mutations. Here we describe a simple protocol for incorporating synthetic oligonucleotides that encode designed, site-specific mutations by assembly PCR. This protocol enables a researcher to “hedge the bets,” namely, to explore a large numbe
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查看完整版本: Titlebook: Directed Evolution Library Creation; Methods and Protocol Elizabeth M.J. Gillam,Janine N. Copp,David Ackerle Book 2014Latest edition Spring