BUCK
发表于 2025-3-26 23:23:31
Elizabeth M.J. Gillam,Janine N. Copp,David AckerleIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
FLIT
发表于 2025-3-27 02:31:54
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palette
发表于 2025-3-27 08:26:37
Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolutiond the need to create an effective library from those variants. Generation of a maximally diverse gene library is particularly important when employing nontargeted mutagenesis strategies such as error-prone PCR (epPCR), which seek to explore very large areas of sequence space. Here we present compreh
情感
发表于 2025-3-27 13:20:19
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开始从未
发表于 2025-3-27 16:44:33
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贪婪性
发表于 2025-3-27 18:06:02
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爱国者
发表于 2025-3-27 22:24:27
Generation of Effective Libraries by Neutral Drift accessible sequence space by repeated rounds of mutagenesis and selection to maintain wild-type function. Mutations that are largely neutral for the native function accumulate, and those that are highly detrimental are purged, yielding a library of high diversity and quality. This technique is usef
种属关系
发表于 2025-3-28 03:44:25
Site-Saturation Mutagenesis by Overlap Extension PCRorporation of degenerate base combinations at specific codon locations, giving rise to a precise series of amino acid substitutions in the encoded protein. Here we describe a simple and efficient overlap PCR protocol for the introduction of degenerate bases at either single or multiple codon locatio
Flawless
发表于 2025-3-28 07:48:04
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急急忙忙
发表于 2025-3-28 11:51:17
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