BUCK 发表于 2025-3-26 23:23:31
Elizabeth M.J. Gillam,Janine N. Copp,David AckerleIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the expertsFLIT 发表于 2025-3-27 02:31:54
http://reply.papertrans.cn/29/2807/280663/280663_32.pngpalette 发表于 2025-3-27 08:26:37
Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolutiond the need to create an effective library from those variants. Generation of a maximally diverse gene library is particularly important when employing nontargeted mutagenesis strategies such as error-prone PCR (epPCR), which seek to explore very large areas of sequence space. Here we present compreh情感 发表于 2025-3-27 13:20:19
http://reply.papertrans.cn/29/2807/280663/280663_34.png开始从未 发表于 2025-3-27 16:44:33
http://reply.papertrans.cn/29/2807/280663/280663_35.png贪婪性 发表于 2025-3-27 18:06:02
http://reply.papertrans.cn/29/2807/280663/280663_36.png爱国者 发表于 2025-3-27 22:24:27
Generation of Effective Libraries by Neutral Drift accessible sequence space by repeated rounds of mutagenesis and selection to maintain wild-type function. Mutations that are largely neutral for the native function accumulate, and those that are highly detrimental are purged, yielding a library of high diversity and quality. This technique is usef种属关系 发表于 2025-3-28 03:44:25
Site-Saturation Mutagenesis by Overlap Extension PCRorporation of degenerate base combinations at specific codon locations, giving rise to a precise series of amino acid substitutions in the encoded protein. Here we describe a simple and efficient overlap PCR protocol for the introduction of degenerate bases at either single or multiple codon locatioFlawless 发表于 2025-3-28 07:48:04
http://reply.papertrans.cn/29/2807/280663/280663_39.png急急忙忙 发表于 2025-3-28 11:51:17
http://reply.papertrans.cn/29/2807/280663/280663_40.png