Titlebook: Virus-Induced Gene Silencing in Plants; Methods and Protocol Vincent Courdavault,Sébastien Besseau Book 2020 Springer Science+Business Medi

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Virus-Induced Gene Silencing in Olive Tree (Oleaceae),nd extended period for the production of healthy transformed offspring, among others. Virus-induced gene silencing (VIGS) is an alternative approach to transiently knock out an endogenous gene of interest (GOI) by the introduction of viral sequences encompassing a fragment of the GOI and to exploit

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,ALSV-Based Virus-Induced Gene Silencing in Apple Tree (, × , L.),lines, especially in plant species difficult to transform and challenging to regenerate such as perennial woody plants. In apple tree, a VIGS vector has been previously developed based on the . (ALSV) and an efficient inoculation method has been optimized using biolistics. This report described deta

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Virus-Induced Gene Silencing for Functional Analysis of Flower Traits in Petunia,lysis without generating stable transgenic plants. The . (TRV) is a popular vector for VIGS because it has a wide host range that includes . (petunia), and it induces minimal viral symptoms. Using reporter genes like chalcone synthase (.) in tandem with a gene of interest (GOI; pTRV2-.-GOI), it is p

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Virus-Induced Gene Silencing in Rose Flowers,ethodology of graft-accelerated VIGS in rose aimed at obtaining posttranscriptional gene silencing in the flower. The resulting phenotype can be observed within 5–6 weeks post infiltration. By using this method, we successfully silenced the expression of several genes involved in processes such as s

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Virus-Induced Gene Silencing (VIGS) in Flax (, L.) Seed Coat: Description of an Effective Procedureablished but mainly for gene expression study in vegetative tissue. Here, we present the critical steps of an optimized procedure of VIGS in flax (. L.) seed coat using the . gene as a selectable marker. The present protocol may serve as an effective tool for functional characterization of genes inv

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Virus-Based microRNA Silencing in Plants,NAs) in plants. The VbMS system was originally developed in . and tomato (.) and has been extended to various other plant species such as ., cotton, and wheat with different virus vectors. VbMS is generally designed to use virus vectors to direct the expression of miRNA target mimic (TM) molecules w