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Titlebook: Viral Vectors for Gene Therapy; Methods and Protocol Fredric P. Manfredsson,Matthew J. Benskey Book 2019 Springer Science+Business Media, L

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发表于 2025-3-21 17:48:45 | 显示全部楼层 |阅读模式
书目名称Viral Vectors for Gene Therapy
副标题Methods and Protocol
编辑Fredric P. Manfredsson,Matthew J. Benskey
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Viral Vectors for Gene Therapy; Methods and Protocol Fredric P. Manfredsson,Matthew J. Benskey Book 2019 Springer Science+Business Media, L
描述This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of adeno-associated virus; construction of oncolytic herpes simplex virus; AAV-mediated gene delivery to the mouse liver; and intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and authoritative, .Viral Vectors for Gene Therapy: Methods and Protocols. is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments..
出版日期Book 2019
关键词Recombinant viral vectors; Gene expression manipulation; Genetic modification; Vector delivery; Pharmaco
版次1
doihttps://doi.org/10.1007/978-1-4939-9065-8
isbn_ebook978-1-4939-9065-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Book 2019on troubleshooting and avoiding known pitfalls..Comprehensive and authoritative, .Viral Vectors for Gene Therapy: Methods and Protocols. is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments..
发表于 2025-3-23 01:05:09 | 显示全部楼层
Basic Concepts in Viral Vector-Mediated Gene Therapy potentially prohibitive, especially to the novice gene therapist. In order to aid in this challenging process, here we provide an overview of basic gene therapy modalities and a decision tree that can be used to make oneself aware of the options available to the beginning gene therapist. This infor
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A Scalable Lentiviral Vector Production and Purification Method Using Mustang Q Chromatography and Tcted between 24 and 48 h post-transfection. The supernatant may then be purified by Mustang Q chromatography, concentrated by Tangential Flow Filtration, and finally diafiltered into the final formulation buffer of choice. Here we describe a pilot scale method for the manufacture of a Lentiviral vec
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