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Titlebook: Viral Metagenomics; Methods and Protocol Vitantonio Pantaleo,Laura Miozzi Book 2024Latest edition The Editor(s) (if applicable) and The Aut

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Determination of the Mycovirome of a Necrotrophic Fungus,ated . strains, the extraction of total RNA from . strains for NGS, the bioinformatics pipeline designed and followed to detect mycoviruses in the sequenced samples, and the validation of the in silico detected mycoviruses by different approaches.
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Enrichment, Sequencing, and Identification of DNA Bacteriophages from Fecal Samples,stinal viromes is still very challenging, both experimentally and analytically. This chapter proposes a simple and reproducible protocol to separate and enrich DNA phage particles from fecal samples, to sequence them, and finally obtain a basic but robust bioinformatic characterization and classification of the global bacteriophage community.
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Quentin Lamy-Besnier,Julian R. Garneau experiments reported here show that lactic acid production by bacteria from salivary sediment is reduced by arginine. This supports the hypothesis that arginine acts directly or indirectly on glycolytic processes in oral bacteria and has more than a simple buffering effect on the pH of dental plaque.
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Giulia Tarquini,Simone Maestri,Paolo Ermacora,Marta Martinips were developing similar formations moving toward Marxism-Leninism but not toward the traditional Soviet Union influenced Communist Party USA. Instead they leaned toward China and the Marxism-Leninism of Mao Tse Tung.
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Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophat covers the entire genome with only a limited number of tiled amplicons. The resulting amplicons can be sequenced on all currently available high-throughput sequencing platforms. As an example, we have included a detailed protocol for performing nanopore sequencing and a pipeline for assembling the resulting tiled amplicon data.
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Application of Nanopore-Based Sequencing to Identify Virus Infections in Woody Plants,oupled with its portability, nanopore technology has great application prospects in the field of quick detection of plant viruses. In this protocol, we expose in detail the application of cDNA-PCR nanopore-based sequencing for the detection of plant viruses.
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