Titlebook: Vibrio Cholerae; Methods and Protocol Book 2018

gonioscopy

pericardium

,, as a Native Host Model for Understanding Pathophysiology of ,, a variety of aquatic animals, including vertebrate fish. Here we describe the use of zebrafish (.) as a model for the pathogenic life cycle of .. Being that fish are natural hosts for ., the model provides several significant advantages over existing mammalian models that are not natural hosts.

Intersect

贸易

Isolation of Outer Membrane Vesicles Including Their Quantitative and Qualitative Analyses,l cultures. Here we describe methods for crude and density gradient-purified OMV isolation and protocols for control analyses for protein profiling (SDS-PAGE), detection of indicator proteins (immunoblot analysis), lipid profiling (lipid extraction and LC-MS analysis), vesicle size determination (Na

Glaci冰

,Utilization of , as a Model Organism to Screen Natural Product Libraries for Identification of New teric pathogen in high- and medium-throughput screening campaigns to identify antimicrobials with different modes of action. In this chapter, we describe methods for the optimal growth of . in 384-well plates, preparation of suitable microtiter natural product sample libraries, as well as their scre

Definitive

Infant Mouse Model of , Infection and Colonization,ifferent animal models have been used by scientists over the years to study the pathogenesis of cholera. However, the most commonly used is the infant (suckling) mouse model, which has been found to replicate important aspects of human intestinal colonization. Here we present a detailed protocol for

对手

oxidant

Aspiration

Natural Cotransformation and Multiplex Genome Editing by Natural Transformation (MuGENT) of ,,atural transformation (MuGENT), which allows the generation of strains with multiple scarless mutations in a single step. Here, we provide a detailed description of the methods used for mutagenesis of the cholera pathogen . with a particular emphasis on mutagenesis via MuGENT.

Angiogenesis