Titlebook: Vectors as Tools for the Study of Normal and Abnormal Growth and Differentiation; Heinz Lother,Rudolf Dernick,Wolfram Ostertag Book 1989 S

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New Retroviral Models for Gene Therapy: Swords into Plowshares,RB) gene in retinoblastoma and osteosarcoma tumor cells. In both cases, transgene expression was accompanied by phenotypic changes in the target cells and organ. Established 208F rat fibroblasts expressing the NGF transgene and secreting active NGF were able to bring about the protection of choliner

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Hemopoietic Regulation Assessed in Clonal Culture: A Brief Overview,ty to proliferate and produce progenitors that are committed to differentiation in single lineages. Because of this dynamic nature of the hemopoietic system, studies of the regulatory mechanisms of the hemopoietic system have been carried out by using functional assays. Both the spleen colony assay

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Haemopoietic Cells as Targets for Gene Transfer,itioned hosts. In addition, methods are available for the manipulation of blood-forming cells in vitro. For these reasons haemopoietic cells are potential targets for therapeutic gene transfer. While this goal remains to be achieved, genes have already been successfully transferred to cultured cells

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,Human β-Globin Expression in Murine Bone Marrow Transplant Recipients Reconstituted with Retroviralr novel therapies in human medicine. Fundamental to such therapeutic endeavours is the development of retroviral vectors for efficient infection of somatic cells and the regulated expression of the inserted gene. Here, we will describe the basic features of the retroviral mediated gene transfer syst

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Using Embryonal Stem Cells to Introduce Mutations into the Mouse Germ Line,entified by test-breeding, and their progeny carry the mutation in all their cells. Here I review our work on the isolation of an ES cell line, its use to produce mice deficient in the enzyme HPRT, and recent experiments which demonstrate how the approach can be generalised to introduce other genetic changes into the mouse germ line.

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The Albino Perinatal Lethal Mutation: Identification of Affected mRNAs and Mapping of the Locus by f the normal gene product. In addition, as a prelude to cloning the perinatal survival locus, a long-range restriction map has been established onto which the extents of albino locus deletions are being placed.

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Mutations in Transgenic Mice, have been induced using the different methods of generating transgenic mice. In this article we will summarize our studies on recessive virus-induced insertional mutations and on the induction of dominant lethal mutations by introducing mutant genes into the early embryo.