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Titlebook: Vasculogenic Mimicry; Methods and Protocol Emily Marques dos Reis,Fernanda Berti Book 2022 Springer Science+Business Media, LLC, part of Sp

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Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry In Vitro period. Test agents can be introduced during growth of the 3D cultures to determine their effect on vasculogenic mimicry formation. Here, we describe the protocol for evaluation of the inhibitory effect of drugs on vasculogenic mimicry in vitro using bright-field and fluorescence microscopy on 3D cultures of tumor cells grown in Matrigel.
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Methods in Molecular Biologyhttp://image.papertrans.cn/v/image/980799.jpg
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Vasculogenic Mimicry—An Overviewy aggressive malignant tumor cells, adequate oxygen and nutrient supply for tumor growth and subsequent metastasis process and its presence are related to poor prognosis in patients. VM is independent of endothelial cells, which may partly explain why angiogenesis drug inhibitors have not achieved the expected success for cancer treatment.
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Daniel Delgado-Bellido,Angel Garcia-Diaz,Francisco Javier Olivermeaning are more fragile, and only disciplined effort can maintain a minority language when daily activities impose that of the majority. Nevertheless, languages have enormous symbolic meaning. As sociolinguist Joshua Fishman has written,
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