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Titlebook: Vascular Tissue Engineering; Methods and Protocol Feng Zhao,Kam W. Leong Book 2022 The Editor(s) (if applicable) and The Author(s), under e

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On-Site Differentiation of Human Mesenchymal Stem Cells into Vascular Cells on Extracellular Matrix and mortality worldwide. However, limited sources of functional vascular cells remain a major obstacle in vascular tissue engineering. Here we describe a novel approach whereby functional vascular cells were obtained by on-site differentiation of human mesenchymal stem cells on a vascular extracell
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In Situ Fabrication and Perfusion of Tissue-Engineered Blood Vessel Microphysiological System,del disease states in vitro. We developed a novel device to fabricate arteriole-scale human endothelialized TEBVs in situ with smaller volumes and higher throughput than previously reported. Both primary and induced pluripotent stem cell (iPSC)-derived cells can be used. Four collagen TEBVs with 600
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Engineering Vascular Grafts with Multiphase Structures,fabrication technique used to produce nano- to microfibrous architectures capable of mimicking native extracellular matrix structure. Likewise, polycitrate elastomers are favorable luminal materials for vascular applications because of their hemocompatibility and mechanical properties. Here we descr
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Fabrication of Small-Diameter Tubular Grafts for Vascular Tissue Engineering Applications Using Mulrein, we describe the methodology for fabricating two different types of tubular silk scaffolds aimed for vascular grafting. The first method emphasizes the use of very thin (10–15μm) silk films with unidirectional longitudinal micro-patterns, followed by their sequential rolling, which results in a
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Controlling Pore Size of Electrospun Vascular Grafts by Electrospraying of Poly(Ethylene Oxide) Miccate porous vascular grafts with fibrous morphology closely mimicking the natural extracellular matrix (ECMs). However, the inherently small pore sizes of electrospun vascular grafts often inhibit cell infiltration and impede vascular regeneration. Here we describe an effective and controllable meth
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