Titlebook: Vascular Morphogenesis; Methods and Protocol Domenico Ribatti Book 2021Latest edition Springer Science+Business Media, LLC, part of Springe

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High-Resolution Confocal Imaging of Pericytes in Human Fetal Brain Microvessels,om human fetal brain in high resolution laser confocal microscopy. This method enables to obtain three-dimensional images of pericytes and provides insights about their distribution and localization in the microvessels of human developing brain.

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In Vivo Vascular Network Forming Assay,his method on-demand for testing different cell sources, perturbation of gene functions, growth factors, and pharmaceutical molecules, and for the development and investigation of strategies to enhance neovascularization of engineered human tissues and organs.

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Microfluidic Device Setting by Coculturing Endothelial Cells and Mesenchymal Stem Cells,ilized. Here, we describe a microfluidic cell culture platform for the construction of microvascular networks with supportive perivascular cells. Our results showed that microvascular networks covered by pericyte-like perivascular cells formed in a microfluidic device and their structures were maintained for at least 3 weeks in vitro.