| 书目名称 | Vascular Hyperpermeability |
| 副标题 | Methods and Protocol |
| 编辑 | Binu Tharakan |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | .This volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in vitro; evaluation of barrier integrity used in a two-layered microfluidic device mimicking the blood-brain barrier; isolation and culture of human umbilical vein endothelial cells; measurement of blood-brain barrier hyperpermeability using Evans Blue extravasation assay; lymphatic vascular permeability determined from direct measurements of solute flux; intravital imaging of leukocyte-endothelial interaction by intravital multiphoton microscopy; and evaluation of tight junction integrity in brain endothelial cells using confocal microscopy. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Thorough and cutting-edge, .Vascular Hyperpermeabilit |
| 出版日期 | Book 2024 |
| 关键词 | junctional proteins; fluorometric measurement; confocal microscopic imaging; cytoskeletal disorganizati |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-0716-3429-5 |
| isbn_softcover | 978-1-0716-3431-8 |
| isbn_ebook | 978-1-0716-3429-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media, LLC, part of Springer Nature 2024 |
发表于 2025-3-21 17:53:27
