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Titlebook: Vascular Disease; Molecular Biology an Andrew H. Baker Book 1999 Humana Press 1999

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楼主: Callow
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Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cellsm phosphorylates myosin light chains, thereby playing an essential role in agonist-mediated endothelial cell contraction, paracellular gap formation and increased vascular permeability. We are hopeful that this step-by-step approach will help the reader to understand the discussed methods.
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Nuclear Run-On Assay to Study Gene Transcription in Vascular Cellsription machinery for synthesis of mRNA. Therefore, it is regarded as the gold-standard measurement of overall transcriptional activity of a specific promoter. Other methods of measuring gene transcription, e.g., based on RT-PCR or pulse-labeling of nuclear RNA (.,.), are difficult to perform and are seldom used.
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Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysisr relationship to the presence of disease. Since total genomic DNA usually cannot be analyzed directly, target sequences are amplified by the polymerase chain reaction (PCR). Several methods have been reported that allow detection of small changes in DNA sequence (.). Among them, one of the most com
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