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Titlebook: Vaccinia Virus and Poxvirology; Methods and Protocol Stuart N. Isaacs Book 2012Latest edition Springer Science+Business Media, LLC 2012 Vac

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Screening for Vaccinia Virus Egress Inhibitors: Separation of IMV, IEV, and EEV, and attractive target for antiviral development is viral egress. Part of understanding the mechanism of action of viral egress inhibitors involves determining which virion form is being made. This can be accomplished through buoyant density centrifugation.
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Mousepox, A Small Animal Model of Smallpox,d animals. In addition, several procedures are described for assessing the host response to infection: for example, measurement of virus-specific CD8 T cells and the use of ELISA and neutralization assays to measure orthopoxvirus-specific antibody titers.
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1064-3745 ation advice from the experts.Includes supplementary materia.Since the first edition of .Vaccinia Virus and Poxvirology: Methods and Protocols. was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vacc
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Isolation of Recombinant MVA Using F13L Selection,enes and the F13L with the genome of an F13L knockout virus. Subsequently, selection of virus recombinants is carried out by serial passage and/or plaque purification of viruses that have recovered the F13L gene.
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