Titlebook: Vaccinia Virus; Methods and Protocol Jason Mercer Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 CRISPR.Viral

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Assessing the Structure and Function of Vaccinia Virus Gene Products by Transient Complementation, poxvirus was variola virus, the etiological agent of smallpox; today, poxviruses remain of biomedical significance, both as pathogens and as recombinant vaccines and oncolytic therapies. Vaccinia virus is the prototypic poxvirus for experimental analysis. The 195 kb dsDNA genome contains >200 genes

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Preliminary Screening and In Vitro Confirmation of Orthopoxvirus Antivirals,us (the causative agent of smallpox) as an agent of biowarfare or bioterrorism (Henderson, 283:1279–1282, 1999). In addition to variola, monkeypox, cowpox, and vaccinia viruses are orthopoxviruses of concern to human health (Lewis-Jones, 17:81–89, 2004). Smallpox vaccination, using the closely relat

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Vaccinia Virus Transcriptome Analysis by RNA Sequencing, vaccinia virus and host cell. Here, we describe an RNA-Seq method for analyzing the vaccinia virus transcriptome from virus-infected HeLa cells. We pay particular attention to vaccinia virus-specific aspects of sample preparation, sequencing, and data analyses, but our method could be modified to a

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Ribosome Profiling of Vaccinia Virus-Infected Cells,is method can be used to quantify gene expression at the translational level and precisely pinpoint ribosome loading onto mRNA with codon-level resolution. Genome-wide regulation of mRNA translation can also be determined if RNA-Sequencing (RNA-Seq) is carried out in parallel. Here, we describe a pr

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High-Content Analyses of Vaccinia Plaque Formation,iter is diluted enough to allow for only few cells of the monolayer to be infected, clonal spread of infection can be detected by observing the lesion in the cell monolayer or using virus-specific staining methods. Beyond simple titration, plaque formation bares priceless underlying information abou

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Super-resolution Microscopy of Vaccinia Virus Particles,structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM) to detect fluorescently tagged or immunolabeled viral proteins on purified virions. Tens to hundreds of individual virions can be imaged in a single field of view providing data for single-particle aver