Titlebook: Vaccine Technologies for Veterinary Viral Diseases; Methods and Protocol Alejandro Brun Book 2016 Springer Science+Business Media New York

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Recombinant Swinepox Virus for Veterinary Vaccine Development,ed and used extensively. . (SPV) is well suited to develop recombinant vaccines because of its large packaging capacity for recombinant DNA, its host range specificity, and its ability to induce appropriate immune responses.

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Generation and Selection of Orf Virus (ORFV) Recombinants,d as a promising candidate for novel virus vector vaccines. Our recent work demonstrated that those ORFV-based recombinants were able to induce protective, long-lasting immunity in various hosts that are non-permissive for ORFV. In this chapter we describe procedures for the generation, selection, p

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Polycistronic Herpesvirus Amplicon Vectors for Veterinary Vaccine Development,cine. However, herpesvirus-based vaccines have paved the way for DIVA vaccines (discrimination of infected against vaccinated animals), which are particularly desirable for highly contagious livestock diseases that are otherwise combatted by culling of infected animals..In this chapter, we describe

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Analysis of the Cellular Immune Responses to Vaccines,ds intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradient centrifugation and evaluated for their antigen specificity or reactivity towards a vaccine. Here, we describe staining of bovine lymphocytes with pepti

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