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Titlebook: Vaccine Protocols; Andrew Robinson,Graham H. Farrar,Christopher N. Wi Book 19961st edition Humana Press 1996

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书目名称Vaccine Protocols
编辑Andrew Robinson,Graham H. Farrar,Christopher N. Wi
视频video
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Vaccine Protocols;  Andrew Robinson,Graham H. Farrar,Christopher N. Wi Book 19961st edition Humana Press 1996
描述The production of a book devoted to Vaccine Protocols has proved to be a complex task; the variety of procedures required to design, develop, pro­ duce, and assess a vaccine is immense and covers aspects of chemistry, bio­ chemistry, molecular biology, and immunology. Many of the previous volumes in the Methods in Molecular Biology series provide the background required for work on vaccines; of particular interest are the volumes on Immunochemi­ cal Protocols, Practical Molecular Virology, Proteins, and Nucleic Acids. In the volume on Vaccine Protocols, we have tried to cover the latest scientific methods for the design, production, and assessment of vaccines against viruses, bacteria, and parasites. Essentially, the volume is comprised of three basic chapter types. First, a number of chapters describe in detail the development and production of vac­ cines by specific techniques, including genetic manipulation of viruses or bac­ teria to produce live attenuated vaccines or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. Second, a similar number of chapters describe more general techniques that can be used for the formulation, delivery, and asses
出版日期Book 19961st edition
版次1
doihttps://doi.org/10.1385/0896033341
isbn_ebook978-1-59259-588-4Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 1996
The information of publication is updating

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Temperature-Sensitive Mutant Vaccines,ly successful in controlling poliomyelitis are the prime example of this phenomenon. The three live attenuated strains developed by Sabin were derived from wild-type isolates by rapid sequential passage at high multiplicity of infection in monkey tissue in vitro and in vivo, a regimen that yielded v
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Development of Attenuated , Strains that Express Heterologous Antigens,s a system for delivering foreign antigens to the mammalian immune system. DNA capable of driving the expression of heterologous antigens can be introduced into . vaccine strains using a variety of approaches. In general, there are two common methods of expressing a foreign antigen in salmonellae: f
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Chimeric Proteins, several limitations. Large-scale growth of pathogens may be difficult to achieve and is not completely free of risk. Furthermore, purification of relevant antigenic components from such pathogens may be expensive and time-consuming. Another approach is the use of synthetic peptides corresponding to
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The Use of Adjuvants in Experimental Vaccines,preparation because of its extensive use in experimental vaccines in animals, its strong adjuvant effect, and the considerable literature describing its activity. The combination of mineral oil (Bayol 55) and dead mycobacterial organisms produces a specific cellular reaction in experimental animals.
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The Use of Adjuvants in Experimental Vaccines,iety of natural phospholipids or other polar amphiphiles can be used in an aqueous solution of an antigen to form either unilamellar or multilamellar spherules. The antigen(s) may either be lipid-soluble and insert into the artificial lipid bilayer, bmd to the bilayer, or become entrapped inside the
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