Titlebook: Usage-Based Second Language Instruction; A Context-Driven Mul Ian Pemberton Book 2024 The Editor(s) (if applicable) and The Author(s), unde

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pathogenesis rarely relies on secreted exotoxins, and involves the pro-inflammatory endotoxin (lipopolysaccharide). Given the complexity of bacterial pathogenesis, antibody therapeutics are expected to be most efficient upon targeting more than one virulence factor and/or combining different modes o

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Ian Pemberton9; Denis and Gustafsson 1989). The subcellular location of activated GR has been firmly established to be inside the nucleus. In vivo, the hormone-receptor complex interacts with specific DNA sequences, termed glucocorticoid responsive element (GRE; see below and Becker et al. 1986), and elicits tra

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Ian Pembertons since the general parameters of chromatin organization change from one species to another. The chromatin organization of bacterial DNA proves particularly dramatic when introduced into eukaryotes. The nucleosome positioning of recombinant genes is the result of the interaction between the machiner

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Ian Pembertonlasmid vector under the control of an early or late vaccinia virus promoter (.–.), or a bacteriophage T7 promoter/the encephalomyocarditis virus 5′-untranslated leader sequence which would allow a cap independent mechanism of translation of the transcripts (.). Flanking the gene chosen for expressio

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Ian Pemberton a constitutive promoter. Recent literature showed that newly identified promoters of . are promising as well, in addition to pAOX1 and pGAP..In this chapter, the regulation mechanisms of inducible pAOX1 and constitutive pGAP promoters are discussed. In addition, here we present an overview about th

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Ian Pembertonxpression in any organism (.). Some exo- and endoproteases from . (.) and yeast (.), notably, represent harmful molecules for recombinant (“abnormal”) proteins expressed in these systems, and strategies have been developed to counteract potential or actual hydrolytic processes (.,.). Concurrently, t

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ological activity of a protein may be saturated as the expression of the specific protein is increased. Our work has focused on the limiting steps for the secretion of these heterologous proteins expressed at high level in Chinese hamster ovary (CHO) cells. In general, the factors limiting expressio

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Ian PembertonThe early experimental investigations. were concentrated on few times ionized low-Z ions. Recently, the development of electron beam ion trap (EBIT)., electron beam ion source (EBIS). and heavy ion storage ring. has become possible to produce very highly-charged heavy ions (e.g. U . and Xe.). and to