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Titlebook: Ultrastructure Techniques for Microorganisms; Henry C. Aldrich,William J. Todd Book 1986 Plenum Press, New York 1986 Seen.antibody.biology

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发表于 2025-3-21 18:15:39 | 显示全部楼层 |阅读模式
书目名称Ultrastructure Techniques for Microorganisms
编辑Henry C. Aldrich,William J. Todd
视频video
图书封面Titlebook: Ultrastructure Techniques for Microorganisms;  Henry C. Aldrich,William J. Todd Book 1986 Plenum Press, New York 1986 Seen.antibody.biology
描述The modem microbiologist is often a real specialist who has difficulty under­ standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela­ tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under­ sto
出版日期Book 1986
关键词Seen; antibody; biology; cell; microbiology; microorganism; morphology
版次1
doihttps://doi.org/10.1007/978-1-4684-5119-1
isbn_softcover978-1-4684-5121-4
isbn_ebook978-1-4684-5119-1
copyrightPlenum Press, New York 1986
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Low-Temperature Embedding,ow temperature should reduce the extent of conformational changes (i. e., rearrangement of the peptide chains) that are due to the actions of organic liquids, (3) complete dehydration should be avoided to maintain hydration shells, and (4) the specimen environment should be polar.
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Computer Analysis of Ordered Microbiological Objects, image analysis are needed to extract as much reliable information as possible, for example, about the position of individual protein subunits within an assembly or to give an indication of subunit size and shape.
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X-Ray Microanalysis,be obtained. With the new minicomputer-based systems, biologists can obtain usable results with much less effort, and the technique is thereby more accessible to the average researcher and requires less time investment.
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