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Titlebook: Ubiquitin-Proteasome Protocols; Cam Patterson,Douglas M. Cyr Book 2005 Humana Press 2005 Endoplasmatisches Reticulum.Termination.biochemis

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Purification of E1 and E1-Like Enzymeske proteins required for their subsequent conjugation to cellular targets. The general physical and mechanistic features of the E1 family appear well conserved. Formation of an obligatory E1-ubiquitin thiol ester intermediate forms the basis of a one-step covalent purification of the enzyme on ubiqu
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Assays for RING Family Ubiquitin Ligasescontrol, tumor suppression, and many signaling pathways. Ubiquitination of proteins occurs through an enzymatic cascade of three discrete steps, which results in covalent attachment of ubiquitin to the substrate. The first two steps in this cascade involve the activating and conjugating enzymes, E1
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Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proluding ATPase activity, polyubiquitin-chain binding activity, deubiquitination activity, chaperone-like activity, and substrate remodeling activity. The concerted action of these activities leads to efficient degradation of protein substrates by the 26S proteasome. In this chapter we describe protoc
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