| 书目名称 | Type 3 Secretion Systems | | 副标题 | Methods and Protocol | | 编辑 | Matthew L. Nilles,Danielle L. Jessen Condry | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. Practical and comprehensive, .Type 3 Secretion Systems: Methods and Protocols. is a valuable resource for anyone int | | 出版日期 | Book 2017 | | 关键词 | site-directed mutagenesis; interactions of T3S components; heterologous proteins; effector translocatio | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-6649-3 | | isbn_softcover | 978-1-4939-8260-8 | | isbn_ebook | 978-1-4939-6649-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2017 |
The information of publication is updating
|
|
发表于 2025-3-21 19:59:16
