| 书目名称 | Two-Hybrid Systems |
| 副标题 | Methods and Protocol |
| 编辑 | Luis Oñate-Sánchez |
| 视频video | http://file.papertrans.cn/932/931317/931317.mp4 |
| 概述 | Includes cutting-edge methods and protocols for the study of two-hybrid systems.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the ex |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions. The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful .Methods in Molecular Biology. series, chapters include thekind of detail and key implementation advice that leads to excellent results in the lab. .Authoritative and practical, .Two-Hybrid Systems: Methods and Protocols. serves as an ideal guide to the uses of this ex |
| 出版日期 | Book 2018 |
| 关键词 | Y2H system; Protein-protein interactions; Gene expression; Yeast biology; Protein-DNA interaction; Plant |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-4939-7871-7 |
| isbn_softcover | 978-1-4939-9310-9 |
| isbn_ebook | 978-1-4939-7871-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media, LLC, part of Springer Nature 2018 |
|Archiver|手机版|小黑屋|
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