| 书目名称 | Two-Dimensional Electrophoresis Protocols |
| 编辑 | Raymond Tyther,David Sheehan |
| 视频video | |
| 概述 | Covers a wide biological range of proteome sources including microbes, plants and animals.Addresses key aspects of experimental design.Includes some of the latest approaches such as DIGE and detection |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | The human genome and other large-scale genome sequencing projects have inevitably led to a focus on the proteins encoded by genes. The field of proteomics has grown enormously as a result and a number of high-throughput technologies have now been developed allowing discovery-led investigations of protein populations rather than more traditional hypothesis-led studies on single proteins. These high-throughput techno- gies include gene and protein microarrays, the yeast two-hybrid system, and various mass spectrometry methodologies. However, despite developments and improvements in these technologies, two-dimensional electrophoresis (2DE) remains one of the most widely used approaches. This technique was revolutionised by the development of immobilised pH gradient strips which are now commercially available. This has made possible highly reproducible separations of matched samples. Developments in staining, mass spectr- etry, and bioinformatics supported these developments and have led to a measure of standardisation in design, execution, and analysis of proteomics experiments. This book began life as a proposed update of the excellent volume 2DE Protocols edited by Andrew Link of th |
| 出版日期 | Book 2009 |
| 关键词 | Chromosome; Polyacrylamid-Gelelektrophorese; Proteomics; Translation; algorithms; bioinformatics; biologic |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-59745-281-6 |
| isbn_softcover | 978-1-61737-849-2 |
| isbn_ebook | 978-1-59745-281-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Humana Press 2009 |
发表于 2025-3-21 17:10:28
