| 书目名称 | Two Hybrid Technologies |
| 副标题 | Methods and Protocol |
| 编辑 | Bernhard Suter,Erich E. Wanker |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | .Protein–protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of .Two Hybrid Technologies: Methods and Protocols. is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other .in vivo. methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful .Methods in Molecular Biology™. series format, chapters contain introductions to their respective topics, lists of the necessary material |
| 出版日期 | Book 2012 |
| 关键词 | interactomics; protein-protein interaction detection methods; virus-human cell interactomes; yeast two- |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-61779-455-1 |
| isbn_softcover | 978-1-4939-5811-5 |
| isbn_ebook | 978-1-61779-455-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media, LLC 2012 |
发表于 2025-3-21 20:08:41
