书目名称 | Tissue Proteomics | 副标题 | Pathways, Biomarkers | 编辑 | Brian C.-S. Liu,Joshua R. Ehrlich (Assistant Edito | 视频video | | 概述 | Provides time tested, reproducible laboratory methods and protocols authored by experts in the field, including tips and hints.Presents the most up to date and useful proteomic techniques for profilin | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | With the sequencing of the human genome complete, the field of proteomics, still in its early stages, has become an important and informative field of biomedical research. Many of the protocols and techniques commonly employed by proteomics researchers have been refined over the past several years, while at the same time, new and innovative methods have also been developed. Significantly, many of the protocols used in the study of the human proteome have been developed to examine proteins derived specifically from cell lines, body fluids or human tissues. As a consequence, not all protocols are compatible with the full-range of protein sources commonly used in the laboratory. The current volume on Tissue Proteomics seeks to bring together a number of useful and innovative protocols developed particularly for the proteomic profiling of human tissues. The first two protocols presented in this work focus on high-throughput g- based techniques. Chapter 1 describes 2-D DIGE, a method used to examine the entire proteome within discreet pI fractions, whereas Chapter 2 details several complementary techniques for the specific analysis of glycoproteins found in tissue samples. The next chap | 出版日期 | Book 2008 | 关键词 | Microarray; Termination; biomedicine; cell lines; proteins; proteomics; tissue; transcription | 版次 | 1 | doi | https://doi.org/10.1007/978-1-60327-047-2 | isbn_softcover | 978-1-61737-714-3 | isbn_ebook | 978-1-60327-047-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2008 |
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