| 书目名称 | The Wilms‘ Tumor (WT1) Gene | | 副标题 | Methods and Protocol | | 编辑 | Nicholas Hastie | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts.Includes supplementary material | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume provides a thorough overview of the Wilms’ Tumour Gene (WT1). The book begins with three review chapters that cover the involvement of WT1 in pediatric cancer, kidney disease, and tissue development and homeostasis. The next few chapters discuss cell marking and lineage tracing, epicardial cell methodology, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools. The next group of chapters explores the latest tools in genomics, molecular biology, and biochemistry. They discuss dissecting transcription factor function in cell free systems, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics approaches for analyzing Next Generation Sequence data. The final chapter discusses protocols for clinical trials for immune therapy using anti-WT1 peptides. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding kno | | 出版日期 | Book 2016 | | 关键词 | multifunctional protein; mesenchymal-epithelial switches; zinc finger protein; cell making; lineage trac | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-4023-3 | | isbn_softcover | 978-1-4939-8156-4 | | isbn_ebook | 978-1-4939-4023-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2016 |
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发表于 2025-3-21 16:15:06
