| 书目名称 | The Ubiquitin Code | | 编辑 | Manuel S Rodriguez,Rosa Barrio | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume details novel and popular approaches to decipher the .Ubiquitin Code.. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation .in vitro, a .RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8.R74K .mutant, .in vivo. identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls... ..Authoritative and cutting-edge, .The Ubiq | | 出版日期 | Book 2023 | | 关键词 | label free proteomics; SILAC; Ubiquitin traps; SUMO antibodies; Gly-Gly antibodies | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-2859-1 | | isbn_softcover | 978-1-0716-2861-4 | | isbn_ebook | 978-1-0716-2859-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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发表于 2025-3-21 17:58:19
