书目名称 | The Surfaceome |
副标题 | Methods and Protocol |
编辑 | Kenneth R. Boheler,Rebekah L. Gundry |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions totheir respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and thorou |
出版日期 | Book 2018 |
关键词 | plasma membrane; cell surface proteins; antibodies; lipids and glycans; immunophenotyping markers; avian |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-7553-2 |
isbn_softcover | 978-1-4939-8518-0 |
isbn_ebook | 978-1-4939-7553-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC 2018 |