| 书目名称 | The Nucleus | | 编辑 | Ronald Hancock | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. .The Nucleus, Second Edition .presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and re | | 出版日期 | Book 2015Latest edition | | 关键词 | cell nucleus; intranuclear milieu; nuclei isolation; nucleolus; proteomic analysis | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-4939-1680-1 | | isbn_softcover | 978-1-4939-4873-4 | | isbn_ebook | 978-1-4939-1680-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2015 |
The information of publication is updating
|
|
发表于 2025-3-21 18:21:59
