| 书目名称 | The Molecular Biology of Bacterial Virus Systems | | 编辑 | G. Hobom,R. Rott | | 视频video | | | 丛书名称 | Current Topics in Microbiology and Immunology | | 图书封面 |  | | 描述 | The fact that none of the known DNA polymerases is able to initiate DNA chains but only to elongate from a free 3‘ -OH group raises the problem of how replication is initiated, both at the replication origin and on Okazaki frag ments. It was first shown by A. KORNBERG et al. that a general mechanism to initiate replication is through the formation of an RNA primer catalyzed by RNA polymerases or by a new class of enzymes, the primases (KORNBERG 1980). This mechanism, which can be used in the case of circular DNA molecules or linear DNAs that circularize or form concatemers, cannot be used at the ends of linear DNAs since the RNA primer is removed from the DNA chain, and there is no way of filling the gap resulting at the 5‘ -ends of the newly synthesized DNA chain. In some cases linear DNA molecules contain a palin dromic nucleotide sequence at the 3‘ -end that allows the formation of a hairpin structure which provides the needed free 3‘-OH group for elongation. This mechanism, first proposed by CAVALIER-SMITH (1974) for eukaryotic DNA repli cation, was shown to take place in several systems (KORNBERG 1980, 1982). Another mechanism to initiate replication consists in the specifi | | 出版日期 | Conference proceedings 1988 | | 关键词 | DNA; Elongation; RNA; Termination; bacteria; biology; enzymes; gene; genes; molecular biology; polymer; protein | | 版次 | 1 | | doi | https://doi.org/10.1007/978-3-642-73115-0 | | isbn_softcover | 978-3-642-73117-4 | | isbn_ebook | 978-3-642-73115-0Series ISSN 0070-217X Series E-ISSN 2196-9965 | | issn_series | 0070-217X | | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer-Verlag GmbH, DE |
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发表于 2025-3-21 20:04:54
