| 书目名称 | The ELISA Guidebook | | 副标题 | Second Edition | | 编辑 | John R. Crowther | | 视频video | | | 概述 | A comprehensive survey of proven ELISA methods.Includes detailed instructions to avoid problems during test development.Provides immunological and statistical background.Suitable for scientists at all | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and | | 出版日期 | Book 2009Latest edition | | 关键词 | Antigen; Diagnostic techniques; ELISA; Enzyme linked immunosorbent assays; PCR; Quality control; infectiou | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-60327-254-4 | | isbn_softcover | 978-1-61737-884-3 | | isbn_ebook | 978-1-60327-254-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2009 |
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发表于 2025-3-21 19:35:25
