Titlebook: Systems Chemical Biology; Methods and Protocol Slava Ziegler,Herbert Waldmann Book 2019 Springer Science+Business Media, LLC, part of Sprin

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书目名称Systems Chemical Biology影响因子(影响力)




书目名称Systems Chemical Biology影响因子(影响力)学科排名




书目名称Systems Chemical Biology网络公开度




书目名称Systems Chemical Biology网络公开度学科排名




书目名称Systems Chemical Biology被引频次




书目名称Systems Chemical Biology被引频次学科排名




书目名称Systems Chemical Biology年度引用




书目名称Systems Chemical Biology年度引用学科排名




书目名称Systems Chemical Biology读者反馈




书目名称Systems Chemical Biology读者反馈学科排名

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Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer,010). Although various robust biochemical approaches exist to measure these binding characteristics, analysis of compound binding with isolated targets may not accurately reflect engagement in the milieu of living cells. To realize the influence of cellular context, methods are needed that are capab

发酵剂

Target Engagement of Small Molecules: Thermal Profiling Approaches on Different Levels,f chemical biology. Many different techniques for target identification and engagement are developed, but none of them is generic. Here we describe one of these techniques—the .llular .hermal .hift .ssay (CETSA). The assay works without any labeling of proteins or small molecules, which allows the i

凶兆

Visualizing Bioactive Small Molecules by Alkyne Tagging and Slit-Scanning Raman Microscopy,rows light on their molecular mechanisms of action and specificity. Many studies in this area have employed fluorescence imaging, using molecules of interest labeled with fluorescent dyes. However, modification with a bulky fluorophore may significantly alter the properties, including bioactivity, o

cinder

The Cell Painting Assay as a Screening Tool for the Discovery of Bioactivities in New Chemical Matteening tool for the discovery of new biological activities. The cell painting assay stains various cellular features using six different dyes in one well. By automated image analysis, hundreds of parameters are calculated from the images which deliver a phenotypic profile of the cell. It has been sh

gait-cycle

Proteomic Profiling for Target Identification of Biologically Active Small Molecules Using 2D DIGE, treated with bioactive small molecules. Using such data, a variety of profiling methods have been established for target identification of such bioactive compounds. In this chapter, we describe a proteomic profiling system, ChemProteoBase, based on proteome analysis using two-dimensional difference

ALIEN

Flat-Feet

inspired

Exome Sequencing of Drug-Resistant Clones for Target Identification,ll molecules has been challenging. The gold standard for target identification requires the discovery of mutations in the target protein that block the effects of small molecules in vitro as well as in vivo. Here we describe the procedures for isolating drug resistant clones using the colorectal can