Titlebook: Synthetic Gene Circuits; Methods and Protocol Filippo Menolascina Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2

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Computer-Aided Design and Pre-validation of Large Batches of DNA Assemblies,n the part sequence can cause assembly failures, incurring troubleshooting costs and project delays. As a result, the careful design and checking of the assembly plan is often a bottleneck of large assembly projects, and may require computational support. This chapter demonstrates the use of two fre

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Computer-Aided Planning for the Verification of Large Batches of DNA Constructs,er, both require careful preparation to select restriction enzymes or DNA primers adapted to the expected constructs sequences. In projects involving manufacturing of large batches of synthetic constructs, the task can be tedious and error-prone. This chapter demonstrates the use of two free and ope

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Steady-State Cell-Free Gene Expression with Microfluidic Chemostats,cell. Recent advances in microfluidic devices allowed cell-free reactions to run under nonequilibrium, steady-state conditions enabling the implementation of dynamic gene regulatory circuits in vitro. In this chapter, we present a detailed protocol to fabricate a microfluidic chemostat device which

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,Prediction of Cellular Burden with Host–Circuit Models,lting . is a widely recognized bottleneck in the design of robust circuits. In this tutorial we discuss the use of computational models that integrate gene circuits and the physiology of host cells. Through various use cases, we illustrate the power of host–circuit models to predict the impact of de

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