Titlebook: Structural Genomics; General Applications Yu Wai Chen,Chin-Pang Bennu Yiu Book 2021Latest edition Springer Science+Business Media, LLC, par

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https://doi.org/10.1007/978-1-0716-0892-0Protein production; Structural analyses; Data management; Protein modeling; Computational tools; Structur

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978-1-0716-0894-4Springer Science+Business Media, LLC, part of Springer Nature 2021

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Yu Wai Chen,Chin-Pang Bennu YiuIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Rational Design and Construction of Active-Site Labeled Enzymesimportant task in structural genomics. To characterize the function of an enzyme at molecular level, placing a reporter on the active site of an enzyme can be a strategy to examine the dynamics of the interaction between enzyme and its substrate/inhibitor. In this chapter, we describe an approach of

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Screening and Production of Recombinant Human Proteins: Ligation-Independent Cloningl-expressed, soluble, and stable protein and one that crystallizes and diffracts to a sufficient resolution for structural determination. At the Structural Genomics Consortium, we opted for the ligation-independent cloning (LIC) method which provides the throughput we desire to produce and screen ma

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Screening and Production of Recombinant Human Proteins: Protein Production in sing LIC. In this chapter we provide a step-by-step procedure of our . system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify which proteins or truncated versions are expressed in a soluble and stable form suitable for structural studies. In addit

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