Titlebook: Structural Biology; Practical NMR Applic Quincy Teng Textbook 2013Latest edition Springer Science+Business Media New York 2013

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NMR Sample Preparation,consuming stage for the success of the planned studies. A similar situation occurs in crystallographic studies; however, a difference is that NMR samples usually require isotope-labeling. Because the cost of .C, .N, or .H source compounds is significantly higher than natural abundant sources, the is

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Practical Aspects,cted, the homogeneity of the magnetic field, the type of experiments chosen, how the data are acquired and subsequently processed, etc. In this chapter, practical aspects related to the experiments are discussed in detail.

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Multidimensional Heteronuclear NMR Experiments,parameters can be utilized. This chapter addresses the general strategy and heteronuclear experiments required to achieve sequence-specific assignments of backbone and side-chain resonances. Practical aspects related to the experiments are also discussed in detail.

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NMR-Based Metabolomics, proteomics) and is rapidly growing as evidenced by the increasing number of publications in this field (Fig. 9.1). It studies the global profiles of metabolites in a biological system (cell, tissue, or organism) under a given set of conditions (Goodacre et al. 2004). Arguably, its history can be tr

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will be of great value to researchers in the field of biocheThis 2nd edition begins with an overview of NMR development and applications in biological systems. It describes recent developments in instrument hardware and methodology. Chapters highlight the scope and limitation of NMR methods. While d

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NMR Sample Preparation,steps are especially NMR-oriented and are emphasized in more detail. Some tips for sample preparation are also provided. It should be noted that there is often a great deal of flexibility in the application of protocols. Hence, it is often possible to alter or adapt a technique to specific needs.