书目名称 | Strain Engineering |
副标题 | Methods and Protocol |
编辑 | James A. Williams |
视频video | http://file.papertrans.cn/879/878613/878613.mp4 |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In .Strain Engineering: Methods and Protocols., powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, .E. coli.-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast .Saccharomyces cerevisiae. as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful .Methods in Molecular Biology™. series fo |
出版日期 | Book 2011 |
关键词 | E; coli; bacterial genomes; chromosomal genes; saccharomyces cerevisiae; transposon mutagenesis; genetic e |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-61779-197-0 |
isbn_softcover | 978-1-4939-5846-7 |
isbn_ebook | 978-1-61779-197-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC 2011 |