书目名称 | Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) |
副标题 | Methods and Protocol |
编辑 | Bettina Warscheid |
视频video | http://file.papertrans.cn/876/875455/875455.mp4 |
概述 | Includes cutting-edge methods and protocols in the MiMB standard format.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.In |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and .Protocols .provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups. These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): .Methods and Protocols .will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.. |
出版日期 | Book 2014 |
关键词 | Cell Culture; Gram negative bacteria; Gram positive bacteria; RNAi-mediated gene knockdown; SILAC labeli |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-1142-4 |
isbn_softcover | 978-1-4939-5257-1 |
isbn_ebook | 978-1-4939-1142-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2014 |