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Titlebook: Spermatogonial Stem Cells; Methods and Protocol Jon M. Oatley,Brian P. Hermann Book 2023 The Editor(s) (if applicable) and The Author(s), u

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发表于 2025-3-21 19:34:46 | 显示全部楼层 |阅读模式
书目名称Spermatogonial Stem Cells
副标题Methods and Protocol
编辑Jon M. Oatley,Brian P. Hermann
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contain key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Spermatogonial Stem Cells; Methods and Protocol Jon M. Oatley,Brian P. Hermann Book 2023 The Editor(s) (if applicable) and The Author(s), u
描述This volume explores the latest techniques used in the field of mammalian germ cell biology to study key experimental approaches and perspectives for critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool.  Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding common pitfalls..Cutting-edge and comprehensive, .Spermatogonial Stem Cells: Methods and Protocols. is a valuable resource for both new and experiences investigators looking to expand their knowledge in this developing field.  .
出版日期Book 2023
关键词mammalian gem cell biology; Immunostaining; Cytotoxic Regeneration; Conditional Knockout; RNAi Knockdown
版次1
doihttps://doi.org/10.1007/978-1-0716-3139-3
isbn_softcover978-1-0716-3141-6
isbn_ebook978-1-0716-3139-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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978-1-0716-3141-6The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Jon M. Oatley,Brian P. HermannIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contain key notes and implementation advice from the experts
发表于 2025-3-22 15:26:55 | 显示全部楼层
Viral Transduction of Mammalian Spermatogonial Stem Cells,mising emerging tools for in vivo SSC transduction that are less invasive, compared to lentivirus, since AAV DNA is not integrated into the host genome and the host genome remains intact. In this chapter, we describe protocols using lentiviral and adeno-associated viral vectors to transduce SSCs in vitro and vivo, respectively.
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,Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell: Part 2—Computational Analysis o genomic regions. In addition, we describe Chromatin State Discovery and Characterization (ChromHMM) methodology to integrate data from these individual analyses, plus that from RNA-seq analysis of gene expression, to obtain the most comprehensive overall assessment of epigenetic programming associa
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