Titlebook: Species Diagnostics Protocols; PCR and Other Nuclei Justin P. Clapp Book 1996 Humana Press 1996

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PCR Detection of HIV,n induce, after a long clinically silent period, the acquired immune deficiency syndrome (AIDS). In the case of HIV-1 infections, the majority of individuals will finally suffer from this late disease; whereas HIV-2 infections appear to exhibit a lower frequency of progression to AIDS.

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The Identification of Dengue Virus Using PCR,destroyed by treatment with non-ionic detergent (..) or low osmotic shock. Four antigenically related but distinct serotypes of dengue virus (DEN1, DEN2, DEN3, and DEN4) are circulating in tropical countries and cause dengue fever and dengue hemorrhagic fever, both of which are highly prevalent.

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Isolation and Purification of Plant Nucleic Acids,ive isolation of plant nucleic acids in a purified state. Isolation of DNA from plant tissue must be simple, rapid, inexpensive, reproducible, and efficient, particularly when many samples are required, e.g., in population studies. The increasing use of polymerase chain reaction (PCR)-based technolo

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Isolation and Purification of Insect DNA,of DNA from other organisms will usually work with insect tissue. Our intention in this chapter is to present methods that have worked well in our laboratory, focusing on simplicity, speed, and safety.

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Molecular Identification of Phytopathogenic Viruses, and the diseases they induce diagnosed. These methods are basically the same as those used in other fields of biology and, therefore, some details of their description may appear repetitious, if not cumbersome. However, the current literature indicates that a direct transfer without adjustment of t

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